RNA molecules play crucial roles in various biological processes such as transcription, splicing, translation, and post-transcriptional gene regulation. The stability and function of RNA molecules depend on their chemical composition and structural features. Modified nucleotides have been introduced to RNA to improve their stability and function. Among these modified nucleotides, 2'-TBDMS-rC(Ac) Phosphoramidite has been extensively used to modify RNA molecules. However, the effect of this modification on RNA stability and function has not been fully elucidated.
RNA stability is an important factor that determines the biological activity of RNA molecules. The 2'-TBDMS-rC(Ac) Phosphoramidite modification has been reported to enhance the stability of RNA molecules by increasing their resistance to nuclease hydrolysis. It has been shown that this modification improves the resistance of RNA molecules to both ribonucleases such as RNase A and RNase T1, and chemical nucleases such as piperidine. One of the mechanisms by which this modification enhances RNA stability is by protecting the 2'-OH group of RNA from nucleophilic attack. This modification also prevents the formation of secondary structures in RNA molecules, which can promote the stability of the RNA.
RNA function is determined by its structural features, including secondary and tertiary structures, binding affinity, and enzymatic activity. The 2'-TBDMS-rC(Ac) Phosphoramidite modification has been shown to have a minimal effect on RNA function. This modification is compatible with various RNA enzymes and has been used to modify ribozymes, RNA aptamers, and siRNAs without compromising their function. For example, the modification has been used to improve the activity and stability of hammerhead ribozymes, which are catalytic RNA molecules that cleave other RNAs. This modification has also been used to improve the binding affinity and specificity of RNA aptamers, which are RNA molecules that can bind to specific targets with high affinity and specificity.
In summary, the 2'-TBDMS-rC(Ac) Phosphoramidite modification enhances the stability of RNA molecules by increasing their resistance to nuclease hydrolysis and preventing the formation of secondary structures. This modification has a minimal effect on RNA function and has been used to improve the activity and stability of RNA enzymes and aptamers. Therefore, this modification is a useful tool for RNA modification and research.